Nextseq 550 user guide.

Nextseq 550 user guide NExTSEq 550 SySTEm Streamlined NGS workflow The NextSeq 550 System is part of a fully integrated workflow that spans from library preparation to data analysis, report generation, and data sharing ( Figure 2). Part # 15046563 Rev. The NextSeq 550 System features dual output NextSeq 500/550 NextSeq 1000/2000. nextseq550-500 NextSeq 550 User Guide (document # 15069765) Provides an overview of the NextSeq 550 next generation sequencing instrument components, instructions for operating the instrument, and maintenance and troubleshooting procedures. 5 Reagent Kitのフローセルの室温配送について保管/ 配送情報を更新。NextSeq v2. 2 billion a: Maximum read length: 2 x 150 bp: 2 x 150 bp: 2 x 150 bp: Informatics options: On-Premises Server All DRAGEN pipelines except metagenomics. X; How to set up a PhiX validation run on the NextSeq 500/550 in manual mode 《NextSeq自定义引物指南》（文档号15057456）提供有关使用自定义测序引物代替Illumina测序引物的信息。《NextSeq500系统指南》（文档号15046563）或《NextSeq 550系统指南》（文档号 15069765）提供仪器组件概述、仪器操作说明以及仪器维护和故障排除程序。 BaseSpace帮助 Clusters Passing Filter. Knowledge Base, User Guide, and Illumina Technical Support. Download the System Suite installer from the Illumina website and save it to a network NextSeq 500 and NextSeq 550 Sequencing Systems. TM. B. Dependinguponlibrarytypeandexperience,2– 5 µloflibraryproducesoptimalresults. Sigma-Aldrich, catalog # P7949. 2 に更新する必要が Le système NextSeq 550 fait partie d’un flux de travail entièrement intégré allant de la préparation des librairies à l’analyse des données, la génération de rapports et le partage des données (figure 2). Microlab STAR Line Operator’s Manual, Hamilton Doc ID 624668 Manual Post-Run Wash Duration: 90 minutes Flushes the system with a user-supplied wash solution of laboratory-grade water and Tween 20 (buffer wash cartridge) and 0. Exome sequencing analyzes the protein-coding region of the genome, as a cost-effective alternative to whole-genome sequencing. Illumina® NextSeq™ 550 시스템은 단일 솔루션으로 대용량 시퀀싱(high-throughput sequencing)과 어레이 스캐닝(array scanning)을 지원합니다. 시퀀싱 X 대용량시퀀싱— NextSeq 550은엑솜(exome), 전장유전체(whole-genome) 및전사체(transcriptome) 시퀀싱을 u NextSeq 500Systemhandbuch(Dokument-Nr. 1; Run setup modes for the NextSeq 500/550 System overview and instructions for operating and maintaining the NextSeq 500 and NextSeq 550. El sistema NextSeq 550 incluye un monitor de pantalla táctil, una barra de estado y tres 仅凭Illumina®NextSeq™550系统这一款解决方案，即可实现高通量测序与阵列扫描之间的无缝过渡。测序功能 u 高通量测序—NextSeq550可实现外显子组测序、全基因组测序及转录组测序，并支持TruSeq™、 TruSight™和Nextera™文库。 Embgenix™ PGT-A Kit (RUO) User Manual for Illumina® NextSeq® 500/550 System (080122) takarabio. Assurez-vous que tous les membres du personnel ont reçu une formation sur l’utilisation correcte de l’instrument et sont conscients des éventuels risques pour la sécurité. AmpliSeq Página 29: Introducción De Los Parámetros De Experimento Y Análisis En Ncs (Modo De Experimento Manual) Guía del sistema NextSeq 550 Purge consumables for this run (Purgar consumibles para este experimento): cambie el ajuste para purgar los consumibles de forma automática tras el experimento actual. C April 2014 Updated software descriptions to NextSeq Control Software v1. Instructions for sequencing with custom primers on the NextSeq 500 and NextSeq 550 Systems. X; How to set up a PhiX validation run on the NextSeq 500/550 in manual mode Assay that enables comprehensive genomic profiling from FFPE tissue and runs on the NextSeq 550 System or NextSeq 550Dx Instrument (in Research Mode) and can batch up to eight samples at a time. Netzspannung. 文件＃20048207 Ver. This document will guide you through the steps of setting up and running sequencing experiments on the NextSeq 550 system. NextSeq 500 and NextSeq 550 Sequencing The NextSeq 550Dx Instrument is intended for targeted sequencing of DNA libraries from human genomic DNA extracted from peripheral whole blood or formalin-fixed, paraffin-embedded (FFPE) tissue when used with in vitro (IVD) diagnostic assays. 2 に更新する必要が Page 1 NextSeq 550Dx Instrument Reference Guide Document # 1000000009513 v00 ILLUMINA PROPRIETARY November 2017 FOR IN VITRO DIAGNOSTIC USE; Page 2 NextSeq 550Dx Instrument Reference Guide This document and its contents are proprietary to Illumina, Inc. Sequencing primer compatibility and recommended library kits for the NextSeq 1000/2000 platforms; Sequencing primer compatibility and recommended library types for the HiSeq X and HiSeq 3000/4000; Sequencing primer compatibility of Illumina libraries and kit types for NextSeq 500/550 and MiniSeq Below you will find brief information for sequencing system NextSeq 500, sequencing system NextSeq 550. Upgrade cost will cover upgrade onsite by FSE. • Corrected NaOCl volume to 3 ml for position 28 of the reagent NextSeq 500 and NextSeq 550 Sequencing Systems Site Prep Guide in Brazilian Portuguese PDF(< 1 MB) May 28, 2020. Maximum output per run a: 120 Gb: 121 Gb: 360 Gb: Maximum reads per run: 400 million: 400 million: 1. NextSeq 550Dx测序仪 b. 5 試薬のサポートを追加。 NextSeq v2. The NextSeq 550Dx Instrument is not intended for whole genome or de novo sequencing. 您好！我是一个经过专门训练的 AI 聊天机器人，旨在帮助您了解 Illumina NextSeq 550 测序系统系统指南。我已经彻底审查了该文档，可以帮助您找到所需的确切信息，或者用清晰简洁的术语解释内容。 Das NextSeq 550 System ermöglicht mit verschiedenen Durchsatzniveaus Anwendern eine optimale Betriebs - effizienz. • Corrected NaOCl volume to 3 ml for position 28 of the reagent Troubleshooting Custom Sequencing Primers selected by mistake in manual mode on the NextSeq 500/550; Troubleshooting Cycle 1 Error, Camera Disabled, Failed to Detect NextSeq 500 and NextSeq 550 Sequencing Systems Custom Primers Guide (15057456) Author: Illumina Subject: Instructions for using custom primers on the NextSeq 500 and NextSeq 550 systems. Seleccione Next (Siguiente). Nepasutiliserdanslecadred’examensdiagnostiques. Washing the instrument using manual wash options; diluted to 0. Created Date: 3/3/2020 3:02:03 PM 《NextSeq自定义引物指南》（文档号15057456）提供有关使用自定义测序引物代替Illumina测序引物的信息。《NextSeq500系统指南》（文档号15046563）或《NextSeq 550系统指南》（文档号 15069765）提供仪器组件概述、仪器操作说明以及仪器维护和故障排除程序。 BaseSpace帮助 This tab is used to set up a NextSeq run. The NextSeq 500 and NextSeq 550 Systems are Illumina's benchtop sequencers that offer a combination of flexibility, accessibility, and affordability for sequencing a wide range of applications, from basic research to clinical diagnostics. . FILE NAME. FAQ. X; How to set up a PhiX validation run on the NextSeq 500/550 in manual mode; How to set up a Wash on the NextSeq 500/550 Video; How to set up a run on the NextSeq 500/550 Video; How to skip the pre run check confirmation on the NextSeq 500/550 Feb 28, 2025 · NextSeq 550 amplicon sequencing solution Download: Application note < 1 MB: Jan 29, 2021: NextSeq 550 exome sequencing solution Download: Application note < 1 MB: Jan 29, 2021: NextSeq 550 RNA-Seq solution Download: Application note < 1 MB: Oct 13, 2021 NextSeq 550 Systemhandbuch Dokument-Nr. System overview and instructions for operating and maintaining the NextSeq 550Dx System. Getting Started. Componentes del instrumento. 引言. Select Perform Wash, and then select Manual Post-Run Wash. * Prepared libraries are loaded into a reagent cartridge and then onto the NextSeq 550 System for se - quencing. 《NextSeq自定义引物指南》（文档号15057456）提供有关使用自定义测序引物代替Illumina测序引物的信息。《NextSeq500系统指南》（文档号15046563）或《NextSeq 550系统指南》（文档号 15069765）提供仪器组件概述、仪器操作说明以及仪器维护和故障排除程序。 BaseSpace帮助 u ハイスループットシーケンス：NextSeq™550DxシステムによるDNAライブラリーのシーケンスを行うことができます。 u Real-TimeAnalysis(RTA)：イメージ処理とベースコーリングを行います。詳細については、57ページの「Real-TimeAnalysis」を参照してください。 Below you will find brief information for sequencing system NextSeq 500, sequencing system NextSeq 550. NovaSeq 6000. Systems configured for manual mode with Run Monitoring and Storage in BaseSpace Sequencing Hub require a sample sheet. General. cBot. Type & Size. Document # 15069765 v08. X; How to set up a PhiX validation run on the NextSeq 500/550 in manual mode NextSeq v2. 的弹性通量，同时其数据质量与当前的NextSeq 550系统保持了相同的高标准。借助优化的试剂化学组成实现高数据质量和低运行成本 NextSeq 1000和NextSeq 2000系统以数十年的专业知识为基础，采用了行业领先的边合成边测序（SBS）化学技术，优化后增强了簇亮度， How to select custom recipes on the NextSeq 500/550 in NextSeq Control Software (NCS) 4. NextSeq NextSeq 1000/2000 Control Software v1. 每次运行的最大产出 a: 120 Gb: 121 Gb: 360 Gb: 每次运行获得的最大读数: 4亿: 4亿: 12亿 a: 最大读长: 2 × 150 bp: 2 × 150 bp: 2 × 150 bp: 信息学选项: 本地服务器除宏基因组学以外的所有DRAGEN流程. NextSeq 500 System Guide (15046563 v07) PDF(8 MB) Oct 24, 2023 · Support Center / downloads / NextSeq 550Dx Instrument Reference Guide. The NextSeq 550 System can sequence 3–12 exomes per run. X; How to set up a PhiX validation run on the NextSeq 500/550 in manual mode; How to set up a Wash on the NextSeq 500/550 Video; How to set up a run on the NextSeq 500/550 Video; How to skip the pre run check confirmation on the NextSeq 500/550 NextSeq 500/550 NextSeq 1000/2000. nextseq550-500 NextSeq™ 550 Sequencing System Datasheet Author: Illumina Subject: The NextSeq 550Dx instrument delivers an FDA-regulated, CE-IVD-marked, high-througput sequencing platform to the clinical and research laboratory. Date. Data Tab. 0 installation instructions; NextSeq 1000/2000 Loading Optimization for XLEAP SBS kits; NextSeq 1000/2000 XLEAP SBS Cartridge Preparation Video; NextSeq 1000/2000 loading optimization for Standard SBS kits; NextSeq 1000/2000 reagents disposal and cartridge recycling tool; Purge settings on the NextSeq The NextSeq 550 System harnesses proven Illumina sequencing by synthesis (SBS) technology to deliver highly accurate data and robust performance for multiple applications. NextSeq. SBS chemistry uses a reversible-terminator method to detect single, fluorescently labeled 有关详细信息，请参见《NextSeq自定义引物指南》（文档号15057456）。更新了有关使用NextSeq500试剂盒v2制备试剂的说明：删除了手动将次氯酸钠和双标签引物装入试剂夹盒的步骤。v2试剂夹盒中已预先注入这些试剂。有关详细信息，请参见《NextSeq500/550试剂 NextSeq 1000/2000 Control Software v1. com Takara Bio USA, Inc. 5 hr for automated workflow ~10. Overview. The NextSeq 550 System features dual output A continuación, encontrará información breve para Sistema NextSeq NextSeq 500, Sistema NextSeq NextSeq 550. Sequencing. Alle hier beschriebenen Gefahren können vermieden werden, indem die Standard-Arbeitsabläufe gemäß NextSeq 500 Systemhandbuch (Teile-Nr. Water, laboratory-grade. 5 フローセルは引き続き以前の保管条件が必要となります。 NextSeq v2. Resource Description NextSeq550Dx ResearchMode InstrumentReference Guide(document# 1000000041922) Providesinstructionsforoperatingtheinstrumentandtroubleshooting Documentation, product files, FAQs, and other support resources for the NextSeq 550 System How to select custom recipes on the NextSeq 500/550 in NextSeq Control Software (NCS) 4. 15041950) u Zubehör-KitmitdenfolgendenKomponenten: u TastaturundMaus u GeschirmtesNetzwerkkabel,CAT 5e Below you will find brief information for sequencing system NextSeq 500, sequencing system NextSeq 550. Innovative technologies. 5 hr for manual workflow: Input quantity: 40 ng DNA and/or 40 ng RNA: Instruments Embgenix™ PGT-A Kit (RUO) User Manual for Illumina® NextSeq® 500/550 System (080122) takarabio. NextSeq 550 System. Instructions for using the NextSeq 550Dx instrument. Cloud NextSeq 500 and NextSeq 550 Sequencing System Guide Translated into Japanese : 15069765-08: System: 5 MB: 2023/9/6: NextSeq 500 and NextSeq 550 Sequencing Systems Site Prep Guide in Japanese : 15045113-06: System: 1 MB: 2023/8/29: NextSeq 550Dx Instrument Site Prep Guide Translated into Japanese : 1000000009869-07: System: 1 MB: 2023/8/25 An off-instrument version is available and compatible with the iSeq 100, MiniSeq, MiSeq, and NextSeq 550 Systems. AmpliSeq May 20, 2024 · As with the NextSeq 550 System, the NextSeq 550Dx Instrument delivers high-quality data with over 75% of bases sequenced yielding a quality score of Q30 or higher (Table 1). . 图1：NextSeq 550系统 - 利用SBS测序化学和用户友好工作流程的最新进展， NextSeq 550系统为外显子组、转录组和靶向重测序等应用提供高质量的结果。图2：NextSeq 550系统工作流程 - NextSeq 550系统提供从样本制备到数据分析的简单、集成的工作流程。 Consultare la la pagina di supporto per NextSeq 550 sul sito Web Illumina per accedere alla documentazione, ai download del software, alla formazione online e alle domande frequenti (FAQ). View Options NextSeq 550Dx Instrument Package Insert (200031448 v00) This document provides a guide for the Illumina NextSeq 550 system. Sommario Capitolo1Descrizionegenerale 1 Introduzione 1 Risorseaggiuntive 2 NextSeq550DxinmodalitàRUO(Solousodiricerca) 2 Componentidellostrumento 3 How to denature and dilute libraries on the NextSeq 500/550; How to enable/disable Illumina Proactive on NextSeq 500/550; How to perform washes on the NextSeq 500/550; How to select custom recipes on the NextSeq 500/550 in NextSeq Control Software (NCS) 4. Typ. 及其附属公司（“Illumina”）专有，仅用于其客户在使用此处所述产品的合规使用，不得用于其他目的。未经Illumina事先书面同意，不得以任何目的使用或散播本文档及其内容 Water,laboratory-grade Generallabsupplier Washingtheinstrument(manual wash) Reagentor spectrophotometric-grade methanolorisopropyl alcohol(99%),100ml bottle Generallabsupplier Cleaningopticscomponents periodicallyandsupportthe objectivecleaningcartridge Airfilter Illumina,catalog# 20063988 Forinstrumentswithanairfilter accessiblefromtherearpanel. 50/60 Hz How to denature and dilute libraries on the NextSeq 500/550; How to enable/disable Illumina Proactive on NextSeq 500/550; How to perform washes on the NextSeq 500/550; How to select custom recipes on the NextSeq 500/550 in NextSeq Control Software (NCS) 4. Il sistema NextSeq 550 comprende un monitor touch screen, una barra di stato e tre scomparti. 시퀀싱 X 대용량시퀀싱— NextSeq 550은엑솜(exome), 전장유전체(whole-genome) 및전사체(transcriptome) 시퀀싱을 NextSeq 550测序仪. X; How to set up a PhiX validation run on the NextSeq 500/550 in manual mode Page 33 NextSeq 500 System Guide Prepare for a Manual Post-Run Wash User-Supplied Consumables Volume and Description • NaOCl 1 ml, diluted to 0. Componenti dello strumento. Bead-basednormalizationprocedurescanbevariable. 0 installation instructions; NextSeq 1000/2000 Loading Optimization for XLEAP SBS kits; NextSeq 1000/2000 XLEAP SBS Cartridge Preparation Video; NextSeq 1000/2000 loading optimization for Standard SBS kits; NextSeq 1000/2000 reagents disposal and cartridge recycling tool; Purge settings on the NextSeq 统相融合。*† 利用NextSeq 550 系统上的芯片扫描，研究人员能够立即使用到这种强大的互补技术，进一步探索或验证通过测序检测到的拷贝数变异。有了NextSeq 550 系统，您可以完成最全面的前沿研究应用目录，同时仪器成本降至最低。NextSeq Manual Software Update. Description Sequencing primer compatibility and recommended library kits for the NextSeq 1000/2000 platforms; Sequencing primer compatibility and recommended library types for the HiSeq X and HiSeq 3000/4000; Sequencing primer compatibility of Illumina libraries and kit types for NextSeq 500/550 and MiniSeq Upgrade NextSeq 500 to NextSeq 550 and enable array scanning of CytoSNP- 850k, CytoSNP-12, HumanKaryomap-12, Infinium MethylationEPIC v1. DATE POSTED. Scanning (NextSeq 550 only) Maintenance. Illumina ，Inc. Download < 1 MB. AmpliSeq NextSeq v2. NextSeq 1000/2000系列测序仪. 4. Sequencing Features High-throughput sequencing —The NextSeq 500 and NextSeq 550 systems enable sequencing of exomes, whole genomes, and transcriptomes and supports TruSeq ™ , TruSight Documentation, product files, FAQs, and other support resources for the NextSeq 550 System How to denature and dilute libraries on the NextSeq 500/550; How to enable/disable Illumina Proactive on NextSeq 500/550; How to perform washes on the NextSeq 500/550; How to select custom recipes on the NextSeq 500/550 in NextSeq Control Software (NCS) 4. X; How to set up a PhiX validation run on the NextSeq 500/550 in manual mode Washing the instrument using the manual post-run wash; diluted to 0. 05% Tween 20 wash solution • RFID Reader - Model # TR-001-44 User Guide (document # 15041950) Provides information about the RFID reader in the instrument, compliance certifications, and safety considerations. 15069765 v07DEU Oktober2021 NextSeq 550System“(SpezifikationenfürdasNextSeq 550-System)aufderIllumina-Website. 2 BeadChip compatible with the NextSeq 550. 仅凭Illumina®NextSeq™550系统这一款解决方案，即可实现高通量测序与阵列扫描之间的无缝过渡。测序功能 u 高通量测序—NextSeq550可实现外显子组测序、全基因组测序及转录组测序，并支持TruSeq™、 TruSight™和Nextera™文库。 troussesdusystèmeNextSeq 500/550 v2(document nº 15058065). Embgenix PGT-A Kit protocol workflow overview for the NextSeq system. 3. It can be used for sequencing of exomes, whole genomes, and transcriptomes, and supports TruSeq and Nextera libraries. 2 and RTA v2. 0. Icon Element. TR-001-44)Benutzerhandbuch(Dokument-Nr. See . HiSeq 1000/2500. 通过BSSH进行 Part # 15046563 Rev. 4 Figure 2. NextSeq 500 and NextSeq 550 Sequencing Systems. Account . 0, and Infinium MethylationEPIC v2. 2 に更新する必要が Im Folgenden finden Sie kurze Informationen zu Sequenzierungssystem NextSeq 500, Sequenzierungssystem NextSeq 550. Page 3 of 26 Table of Figures Figure 1. 550Dx操作手册. mit diesem Gerät können Anwender ein großes Spektrum an Proben in nur einem Lauf sequenzieren: NextSeq. PrepareHT1 1 RemoveHT1from-25°Cto-15 Instructions for using the NextSeq 550Dx Instrument. 5, prepared in previous step; 1x NextSeq™ 500/550 Buffer Cartridge v2 (75 cycles), at room temperature; Set up sequencing run parameters During this step, sequencing run parameters are selected on the NextSeq™ 550. Instrument Administration. X; How to set up a PhiX validation run on the NextSeq 500/550 in manual mode NExTSEq 550 SySTEm Streamlined NGS workflow The NextSeq 550 System is part of a fully integrated workflow that spans from library preparation to data analysis, report generation, and data sharing ( Figure 2). The NextSeq 550 System offers a simple and reliable method to identify coding variants by accurately sequencing even the most difficult genomic regions. 5 Reagent Kitはソフトウェアのバージョンを2. Files. BaseSpace User Guide. 15046564) u RFIDReader(Modell-Nr. Illumina® NextSeq™ 500およびNextSeq 550システムは、ハイスループットシーケンスのパワーと卓上型シーケンス装置の簡易さを兼ね備えています。はじめに • NextSeq 550ハイスループットシーケンス：NextSeq 500 システムおよびシステムはエクソーム、全 Olá! Eu sou um chatbot de IA especificamente treinado para ajudá-lo com o NextSeq 550 Sistema de Sequenciamento Guia do sistema. X; Waste Volumes for NextSeq 550 The NextSeq 550 System harnesses proven Illumina sequencing by synthesis (SBS) technology to deliver highly accurate data and robust performance for multiple applications. 0 installation instructions; NextSeq 1000/2000 Loading Optimization for XLEAP SBS kits; NextSeq 1000/2000 XLEAP SBS Cartridge Preparation Video; NextSeq 1000/2000 loading optimization for Standard SBS kits; NextSeq 1000/2000 reagents disposal and cartridge recycling tool; Purge settings on the NextSeq How to denature and dilute libraries on the NextSeq 500/550; How to enable/disable Illumina Proactive on NextSeq 500/550; How to perform washes on the NextSeq 500/550; How to select custom recipes on the NextSeq 500/550 in NextSeq Control Software (NCS) 4. General lab supplier. How long does a NextSeq 500/550 system check take to complete? How long does it take to purge all reagents in a NextSeq 500/550 run? How many extra cycles are included in NextSeq 500/550 reagent cartridges? How to set up a NextSeq 500/550 to upload to a BaseSpace Enterprise account using NCS v. 12% Loaded onto the reagent wash cartridge (position #28) • 100% Tween 20 Used to make 125 ml 0. X 『NextSeq 500 System Guide（文書番号』：15046563）または『NextSeq 550 System Guide（文書番号』： 15069765） X 『NextSeq System Safety and Compliance Guide』（文書番号：15046564） X 『RFID Reader - Model # TR-001-44 User Guide』（文書番号：15041950） X 付属品キット。これには以下の NextSeq 500 and NextSeq 550 Sequencing Systems Product Documentation. Stromverbrauch. Name. This video will guide you through the necessary steps to take when wanting to perform a Maintenance or Manual Post-Run wash on the Nextseq 500/550 & Dx. X; How to set up a PhiX validation run on the NextSeq 500/550 in manual mode; How to set up a Wash on the NextSeq 500/550 Video; How to set up a run on the NextSeq 500/550 Video; How to skip the pre run check confirmation on the NextSeq 500/550 NextSeq 550系统 ; 仪器服务和咨询 System overview and instructions for operating and maintaining the NextSeq 500. 05%. Failure to find fiducial errors on the NextSeq 1000/2000 NextSeq 1000/2000 Control The NextSeq 550 RNA-Seq solution begins with RNA library preparation using Illumina Stranded Total RNA Prep, Illumina Stranded mRNA Prep, or Illumina RNA Prep with Enrichment. • Removed instructions to add NaOCl to the buffer wash cartridge for a manual wash. Apps Tab on page 24. NextSeq 500 and NextSeq 550 Sequencing Systems Site For more information, refer to the Sequencing Analysis Viewer User Guide (part # 15020619). NextSeq 550Dx Instrument b NextSeq 1000 and NextSeq 2000 Systems. Created Date: 11/7/2017 1:08:36 PM Part # 15046563 Rev. Overlapping and low-quality clusters are removed. • Corrected NaOCl volume to 3 ml for position 28 of the reagent System overview and instructions for operating and maintaining the NextSeq 500. X; How to set up a PhiX validation run on the NextSeq 500/550 in manual mode NextSeq™ 550 Sequencing System Datasheet Author: Illumina Subject: The NextSeq 550Dx instrument delivers an FDA-regulated, CE-IVD-marked, high-througput sequencing platform to the clinical and research laboratory. 2 Manifest File (BPM Format) (GRCh37) The NextSeq 550 RNA-Seq solution begins with RNA library preparation using Illumina Stranded Total RNA Prep, Illumina Stranded mRNA Prep, or Illumina RNA Prep with Enrichment. Leistungsangaben. AmpliSeq Water,laboratory-grade Generallabsupplier Washingtheinstrument(manual wash) Reagentor spectrophotometric-grade methanolorisopropyl alcohol(99%),100ml bottle Generallabsupplier Cleaningopticscomponents periodicallyandsupportthe objectivecleaningcartridge Airfilter Illumina,catalog# 20063988 Forinstrumentswithanairfilter accessiblefromtherearpanel. 1x NextSeq™ 500/550 High Output Flow Cell v2. 2 . SBS uses a reversible-terminator method, with fluorescently labeled nucleotides to detect single bases as they are incorporated into growing DNA strands. Find compatible library prep kits Most Local Run Manager analysis modules will prompt you to select a library prep kit for a given run. Hands-on time ~2. The BaseSpace Support page provides access to the BaseSpace . NovaSeq X/X Plus. Reference Material. On the NextSeq™ 550 Home screen, select Experiment. Support . 12%. Guida del sistema NextSeq 550. Das NextSeq 550 System bietet zudem für zahlreiche, häufig eingesetzte Sequenzierungs - anwendungen eine Durchlaufzeit von einem Tag. 5. 15046563)oderNextSeq 550Systemhandbuch (Dokument-Nr. Public Data Tab on page 24. NextSeq 550 Guidedusystème Documentnº 15069765v07FRA Octobre 2021 EXCLUSIFÀILLUMINA Destinéàlarechercheuniquement. HiSeq X. Spezifikation. Page 12 NextSeq 550Dx Research Mode Instrument Reference Guide Flow Cell Overview Figure 4 Flow Cell Cartridge A Lane pair A—Lanes 1 and 3 B Lane pair B—Lanes 2 and 4 C Flow cell cartridge frame The flow cell is a glass-based substrate on which clusters are generated and the Instructions for customers using the NextSeq 550Dx instrument in the European Economic Area, United Kingdom and Switzerland. Troubleshooting. Denaturing and Diluting Libraries for the NextSeq System (document # 15048776) Index color balancing for the NextSeq 500/550 and MiniSeq systems Instrument Service Contract Plans Instrument maintenance and shutdown procedures for extended site closures †研究者はNextSeq 550システムのマイクロアレイスキャンを活用し、シーケンスで検出されたコピー数変異を詳細に探索または検証する、強力で補完性のあるテクノロジーに即座にアクセスすることができます。NextSeq 550システ View online or download PDF (1 MB) illumina NextSeq 500, NextSeq 550 Guia de usuario • NextSeq 500, NextSeq 550 PDF manual download and more illumina online manuals Manualzz Explorar diretório cells, NextSeq 500 System, NextSeq 550 System, NextSeq 550Dx Instrument in RUO mode, or NextSeq 2000 System. Washing the instrument (manual wash) Reagent or spectrophotometric-grade methanol or isopropyl alcohol (99%), 100 ml NextSeq 1000/2000 Control Software v1. 어레이 스캐닝(NextSeq 550에만 해당) • Control Software에 통합된 어레이 —스캐닝 기능 NextSeq 550은 동일한 기기와 Control Software에서 어레이 스캐닝과 고처리량 시퀀싱 기능을 지원합니다. 9. Figure 2: Example NextSeq 550 System sequencing workflow— The NextSeq 550 System offers a simple, integrated sequencing How to select custom recipes on the NextSeq 500/550 in NextSeq Control Software (NCS) 4. SBS chemistry uses a reversible-terminator method to detect single, fluorescently labeled NextSeq 550 amplicon sequencing solution Download: Application note < 1 MB: Jan 29, 2021: NextSeq 550 exome sequencing solution Download: Application note < 1 MB: Jan 29, 2021: NextSeq 550 RNA-Seq solution Download: Application note < 1 MB: Oct 13, 2021 NextSeq 550 amplicon sequencing solution Download: Application note < 1 MB: Jan 29, 2021: NextSeq 550 exome sequencing solution Download: Application note < 1 MB: Jan 29, 2021: NextSeq 550 RNA-Seq solution Download: Application note < 1 MB: Oct 13, 2021 cells, NextSeq 500 System, NextSeq 550 System, NextSeq 550Dx Instrument in RUO mode, or NextSeq 2000 System. During the run, RTA filters raw data to remove reads that do not meet the data quality threshold. 1. Below you will find brief information for sequencing system NextSeq 500, sequencing system NextSeq 550. Mar 8, 2020. Apps Tab See . NextSeq System Site Prep Guide The Illumina NextSeq 500 and NextSeq 550 systems combine the power of high-throughput sequencing with the simplicity of a desktop sequencing instrument. FAQ NextSeq 500 (référence 15046563_FRA) ou Guide du système NextSeq 550 (référence 15069765_FRA). 1: • Added recipe name NextSeq Mid for use with the NextSeq 500 Mid Output Kit. 15046563_DEU) oder NextSeq 550 Systemhandbuch (Teile-Nr. 本文档及其内容归. 100 bis 240 Volt Wechselstrom bei. Revision History. Table 1: NextSeq 550Dx Instrument performance parameters—Diagnostic Mode a,b Flow cell configuration Read length Output Run time Data qualityc 図1：NextSeq 550システム NextSeq 550システムは、SBS ケミストリーとユーザーフレンドリーなワークフローで最新の技術に対応することにより、エクソーム、トランスクリプトーム、およびターゲットリシーケンスのさまざまなアプ Welcome to the Nextseq 500/550 & Dx instructional video series. Library Preparation. 15069765) u NextSeq-SystemSicherheits-undCompliance-Handbuch(Dokument-Nr. 15069765_ DEU) befolgt werden. NextSeq 550 System Specification Sheet Download: Data sheet < 1 MB: Oct 28, 2022: Explore the transcriptome with single-cell resolution Download: Technical note: 1 MB: Mar 1, 2022: Data Security with Illumina Proactive Download: Technical note < 1 MB: Oct 4, 2022: Multiomic interrogation of the immune system at single-cell resolution Download Instructions for operating and maintaining the NextSeq 500 and NextSeq 550 instruments. El Sistema NextSeq 500 y NextSeq 550 es un secuenciador de próxima generación que ofrece una plataforma de secuenciación flexible para una amplia gama de aplicaciones de investigación, incluyendo genómica, transcriptómica y inmunogenómica. Guía del sistema NextSeq 550. The NextSeq 550 system provides a seamless transition between high-throughput sequencing and array scanning. Figure 2 : Exemple de flux de travail du système de séquençage NextSeq 550 : le système NextSeq 550 propose un flux de The NextSeq 550Dx Instrument is intended for targeted sequencing of DNA libraries from human genomic DNA extracted from peripheral whole blood or formalin-fixed, paraffin-embedded (FFPE) tissue when used with in vitro (IVD) diagnostic assays. The Illumina NextSeq 550 is a single solution that provides a seamless transition between high-throughput sequencing and array scanning. •Ajoutdunomdelaformule NextSeq Midàutiliseraveclatrousse dedébitmoyenNextSeq 500. Mises en garde de sécurité : généralités. Created Date: 11/7/2017 1:08:36 PM NextSeq v2. 13. Close Window. 0 BeadChips. Figure 2: Example NextSeq 550 System sequencing workflow— The NextSeq 550 System offers a simple, integrated sequencing Best practices for low diversity sequencing on the NextSeq 500/550 and MiniSeq systems; 《NextSeq自定义引物指南》（文档号15057456）提供有关使用自定义测序引物代替Illumina测序引物的信息。《NextSeq500系统指南》（文档号15046563）或《NextSeq 550系统指南》（文档号 15069765）提供仪器组件概述、仪器操作说明以及仪器维护和故障排除程序。 BaseSpace帮助 Illumina® NextSeq™ 550 시스템은 단일 솔루션으로 대용량 시퀀싱(high-throughput sequencing)과 어레이 스캐닝(array scanning)을 지원합니다. At Illumina, our goal is to apply innovative technologies to the analysis of genetic variation and function, making studies possible that were not even imaginable just a few years ago. View Options CytoSNP-850K v1. Die NextSeq 500- und NextSeq 550-Systeme sind die Tischsequenzer von Illumina, die eine Kombination aus Flexibilität, Zugänglichkeit und Erschwinglichkeit für die Sequenzierung einer Vielzahl von Anwendungen bieten, von der Grundlagenforschung bis hin zur klinischen Diagnostik. Feb 28, 2025 · NextSeq 550 System Specification Sheet Download: Data sheet < 1 MB: Oct 28, 2022: Explore the transcriptome with single-cell resolution Download: Technical note: 1 MB: Mar 1, 2022: Data Security with Illumina Proactive Download: Technical note < 1 MB: Oct 4, 2022: Multiomic interrogation of the immune system at single-cell resolution Download A NextSeq 500/550 Kit cannot be used for a diagnostic mode run. System overview and instructions for operating and maintaining the NextSeq 500 and NextSeq 550. Public . and its affiliates ("Illumina"), and are intended solely for the contractual use of its customer in connection with the use of the product Troubleshooting Custom Sequencing Primers selected by mistake in manual mode on the NextSeq 500/550; Troubleshooting Cycle 1 Error, Camera Disabled, Failed to Detect 16S rRNA sequencing on NextSeq™ 1000 and NextSeq 2000 Systems Download: Application note: 1 MB: High-plex spatial proteogenomics of FFPE tissue sections Download: Application note: 2 MB: NextSeq™ 1000 and NextSeq 2000 single-cell RNA sequencing solution Download: Application note < 1 MB: May 30, 2024: NextSeq 1000 and NextSeq 2000 RNA Visite la página de asistencia de NextSeq 550 en el sitio web de Illumina para acceder a la documentación, las descargas de software, la formación en línea y las preguntas frecuentes. 及其附属公司（“Illumina”）专有，仅用于其客户在使用此处所述产品的合规使用，不得用于其他目的。未经Illumina事先书面同意，不得以任何目的使用或散播本文档及其内容 Run folder sizes and Disk Space on the NextSeq 500/550; How to enable Proactive on the NextSeq 500/550; Best practices for maintaining the computer on a NextSeq; How to perform a System Check on the NextSeq 500/550; Large image 9; How to Create LRM Credentials to Perform a NextSeq 550 System Check in NCS 4. 12% sodium hypochlorite (reagent wash cartridge). How to denature and dilute libraries on the NextSeq 500/550; How to enable/disable Illumina Proactive on NextSeq 500/550; How to perform washes on the NextSeq 500/550; How to select custom recipes on the NextSeq 500/550 in NextSeq Control Software (NCS) 4. Tween 20. FILE INFO. Já revisei cuidadosamente o documento e posso ajudá-lo a localizar as informações exatas de que precisa ou explicar o conteúdo de maneira clara e simples. NextSeq 500/550 NextSeq 1000/2000. Search. Page. Manifest files, cluster file, and LIMS product descriptor for the CytoSNP-850K v1. rbqly zpyfjpa qsuuush ruugl tanag vupx jqjp fopqw tksbe zaeqgel